Samiksha Kukal

7 Questions 3 Answers 0 Followers

Questions related from Samiksha Kukal

Please suggest me an inducer of PPAR alpha protein expression?

I am in a need of molecule which induces the protein expression of PPARalpha gene. Please suggest.

01 January 2019 8,283 2 View

Can someone tell me estradiol inducible gene in brain endothelial cell lines?

I want to know a gene strongly altered by 17 beta estradiol in human brain endothelial cells, so that i can take that gene as a control for the system.

05 May 2017 2,428 3 View

Could anyone guide me on procedure to dissolve and make dilutions of 17 beta estradiol?

I am trying to perform gene expression experiment with 17 beta estradiol using HepG2 Cell lines. Somehow i am not able to get any induction or akteration. It looks like i am doing something wrong...

01 January 2017 7,274 5 View

Can someone suggest E2-inducible gene in HepG2 cells as an internal control?

I am using HepG2 cells as the cell model. I am giving treatment of estradiol. But i want to be sure that my estradiol drug is dissolved correctly. so anyone can suggest me a known E2 inducible...

01 January 2017 2,261 0 View

For how many days i have to culture cells in phenol red free media before giving E2 treatment?

I am using HepG2 cells as the model system

01 January 2017 9,556 1 View

Can I use the half life of the drug as the time point?

I don't know the time since all individual genes will be expressed at different time points. Can I use half life of the drug as the time point?

01 January 2016 6,175 1 View

How to decide time points of RNA extraction in microarray experiment?

Hello Experts, How do we decide time points of RNA extraction after giving drug to the cell lines for checking the gene expression profile in a microarray?

01 January 2016 6,062 6 View