Objective is to isolate MicroRNA from zebrafish embryo and tissues, and run a transcriptome analysis on it. Most published literature on zebrafish seems to use Trizol based total RNA isolation method. There are also some kits such as mirVANA for specifically isolating the microRNA. I am looking for a kit or a method that is efficient and also cost-effective for my purpose. Would you suggest that total RNA isolation with Trizol is good enough or should I go with microRNA specific kits.
Hi Ankur
I use Qiagen kit for this purpose and Micro RNA concentration was well and It was enough for even northern blot
good lock
Thanks Dinesh. I am aware of these commercially available kits which is why I asked my question. I wanted help chosing the best out of these. If a cheaper total RNA extraction kit works then why would I go for an expensive product.
Hi Ankur,
I don`t know if you already found a good kit for your purpose.
I have used first Trizol and then this kit with great results for zebrafish eggs and tissue: PureLink RNA Micro Scale Kit (Invitrogen).
It was published in DOI: https://doi.org/10.4049/jimmunol.1601958
Kind regards,
Simon
Thanks Simon. I will check this out. We have ordered miRVana kit for microRNA extraction.
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