In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

Defining effective diffusivity in porous zone and non-porous zone?

Hi all, I am working on methanol steam reforming using packed bed for hydrogen generation. I am using FLUENT software for CFD simulation. In reference paper, authors mentioned that effective mass...

10 February 2021 6,104 1 View

Online Shopping Security Survey?

I would appreciate if you answer my DBA research questionnaire. Thank you all in...

10 February 2021 1,990 3 View

Can't run my IPMSM drive system with DC-supply higher than 125 V. Have anyone had similar problem?

Hey, I try to run my 4 pole IPMSM with PWSVM and use a typical controller with PI-controller with a current loop and speed loop. When I run my system with DC-supply 100 V with a steady-state speed...

28 January 2021 7,354 8 View

I am trying to run a simulation on LS-Dyna and I am facing the error below with Nodal rigid constraints. Can anyone tell how to fix it?

*** Error 20393 (STR+393) Nodal rigid body ID 85000010 contains node ID 83500180 already used in a rigid body/nodal rigid body definition.

20 January 2021 2,019 1 View

LC-MS metabolomics data after annotation some have their compound name but no KEGG id or with KEGG id but no pathway. How to interprete?

Recently I have generated LC-MS metabolomics data. After annotation, some have their compound name but either no KEGG id or KEGG id but no pathway. How to interpret those compounds? Additionally,...

18 January 2021 1,938 3 View

How can I get the chromosomal location of a list of genes?

I'm looking for a bioinformatics website to get the chromosomal location of a radish gene id list, but in one shot instead of doing it gene by gene. Is there a way to do this? Thanks

17 January 2021 8,810 3 View

Carboniferous shark teeth - ID help?

Dear everyone! I am looking for some help regarding the identification of two Carboniferous shark teeth from Hungary. See the pic attached. I'd go for Bransonellidae family for A, and Falcatidae...

21 December 2020 1,226 4 View

How to modify an article's "field of expertise" or "subspecialty"?

Greetings; I've recently published a preprint for a COVID treatment RCT, which appeared under "Dental" specialty instead of "Infectious diseases (ID)". This is an issue as it doesn't appear under...

14 December 2020 6,614 4 View

Do you think that the president in Iraq should be able to change the economic situation?

13 December 2020 7,193 3 View