Dear Olubunmi,

you can evaluate this indices very easy compared to expensive and complex methods like HPLC.

You can read some of our manuscripts regarding FRAP Test (The ferric reducing antioxidant power)., its very rigid method with high precise and very easy to do, following i wrote this technique to you, I wish you can use it easily.

FRAP Test:

Total antioxidant capacity of Everything:

The working FRAP reagent will prepare by mixing 10 volumes of 300 mmol/L acetate buffer, pH 3.6, with 1 volume of 10 mmol/L TPTZ (2, 4, 6-tripyridyl-s-triazine, in 40 mmol/L hydrochloric acid) and with 1 volume of 20 mmol/L ferric chloride. You must use all solutions on the day of the experiment. Then, 50 μL of your sample and 150 μL of deionized water add to 1.5 mL of the FRAP reagent. The reaction mixture incubat at 37 ºC for 5 minutes. Finally, samples will centrifuge (1000 g, 1 min) and the absorbance of developed color in the supernatant will measured at 595 nm by  spectrophotometer.

Enclosed you will kindly find our manuscript for using as a valid reference.

Regards,

Mehdi 

Dear

The antioxidant activity is the ability of sample to scavenge and radicals molecules. However their is in vitro and animal models procedures. However HPLC technique is capable to investigate the active ingredient within your sample and their amount. But you can't neglect the in vitro and in vivo studies.

Regards

Why don't you use DPPH spectrophotometry assay

Thank you all