Please I am a doctoral student, does any one has Primer 7 and XLstat softwares and the keys? Please I need them to analyze my environmental data?

More Vincent Happy Ogwugwa's questions See All

How do I compute SEM for percentages using SPSS?

I'm evaluating the reproductive performance of quails. I have the fertility, and hatchability percentages for six different sire groups and would like to determine the significant difference...

14 July 2024 2,200 5 View

How do I fix the MS NET.Framework 1.1.4322 condition for installing PowerMarker software?

I have been trying to install PowerMarker on my Windows 10 PC but it is not working. One of the requirements for PowerMarker installation is MS NET Framework 1.1.4322 condition, however, Windows...

07 June 2024 1,553 1 View

Can we call ‘pressure induced phase transition’ as ‘synthesis?

I would like to explain my question with the following illustrative situation. In general, when we apply the high-pressure to the solid materials at room temperature conditions, the solid...

21 May 2024 1,358 3 View

Window size TSAD (Time Series Anomaly Detection) ?

Dear all, One of the key points in TSAD (Time Series Anomaly Detection) when using a sliding window is the size of the window. For (quasi)-periodic time series, methods such as AutoPeriod,...

13 May 2024 920 3 View

Is there any way to determine the concentration of protein in a gel-ready protein sample?

I am performing western blot and recently i have been obtaining faint bands for the samples i had already run and had got darker bands. I wish to determine the concentration of protein in those...

08 May 2024 8,933 4 View

How to Stabilize ssDNA for PCR?

Hello, my lab currently utilizes a method that uses ssDNA as a template for real time qPCR. We are looking to prep a bunch of PCR plates in advance and run them overnight on our qPCR machine using...

25 April 2024 7,432 1 View

How can the adoption of genotypic approaches in diagnostic microbiology impact antibiotic resistance prevention?

This question stirs further discussion regarding the potential benefits and challenges associated with integrating genotypic approaches in diagnostic microbiology for antibiotic resistance...

07 April 2024 6,560 2 View

Can someone point us to software to identify closely short related protein sequences in other proteins as compared to a target short protein sequence?

Can someone point us towards analysis software that is able to identify similar short protein sequences within a protein database through use of a set of comparisons (e.g., similar charged or...

25 February 2024 508 1 View

Does the structure and nature of the liquid state depend on its initial composition?

I would like to explain my question with the following illustrative situation. In general, when we apply the high-pressure and high temperature to the solid materials, the solid melts and it goes...

21 February 2024 4,573 4 View

In GaussView /Gaussian calculation, Ii get open-new-file error. Why I am getting nad how to resolve?

11 February 2024 6,312 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,667 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,113 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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05 August 2024 9,056 3 View

What researches are there for satisfaction level of the hospital attachment for student nurses ?

I am doing a study on nursing students satisfaction on the hospital attachment whether they are satisfied with the clinical attachment or not. I need more research studies on this

03 August 2024 6,985 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

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01 August 2024 4,672 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,404 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 840 2 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

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25 July 2024 6,517 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

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23 July 2024 1,329 5 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...

20 July 2024 5,404 2 View

Randolph B Caldwell

If software licenses are not available through your school, then I would suggest using free versions. There are plenty out there. For example:

https://www.sas.com/en_us/software/university-edition.html