Please could any one help me with the primer for plant RuBisco genomes in the legumes?

More Catherine V. Nnamani's questions See All

Are there any information about protocols of CRISPR in leishmania strains?

I am looking for a CRISPR protocol for application in leishmania strains

08 July 2024 9,732 1 View

Has anyone successfully used steam on Persicaria perfoliata and its seeds?

I know steam has become a potential for killing invasive plants. I am curious to know whether or not it has been used successfully in killing the plant and the seeds of Mile-a-minute (Persicaria...

19 June 2024 6,216 0 View

Air bubbles forming underneath tissue during dehydration step of IHC?

Hi all, I've processed my own IHC for many years and never run into this problem before, but the last few times I've tried staining, I've had the same issue. I'm wondering if it's a reagent...

08 May 2024 3,979 1 View

Why are there fumes while heating melamine to graphitic carbon nitride ?

I am heating melamine at 550°C for 4 hrs but around 470°C I could observe white fumes from the muffle furnace which deposits as white powder on the outside of the furnace. I do not know why the...

01 May 2024 9,719 2 View

How do I compute for my sample size if the QUANT phase of my Explanatory sequential research is Mediation Analysis?

I am quite confused about what formula to use to compute my sample size. I will be conducting a Sequential Explanatory design wherein my QUANT phase will make use of mediation analysis and my qual...

22 April 2024 5,588 6 View

To what extent does Sysmex DI-60, with automated prep, advanced flagging, and quality control, minimize errors in hematology tests,and enhance result?

This research question delves into the multifaceted impact of integrating the Sysmex DI-60 hematology analyzer with several advanced features—automated sample preparation, advanced flagging...

12 April 2024 1,708 1 View

How to know how much to dilute a sample of acid for ICP OES?

I have solutions from an SFEIS test that I want to check for the dissolved elements. the solution used was Na2SO4 or H2SO4. How much should I dilute the sample before analysis? I am not sure what...

05 March 2024 4,266 4 View

HAs anyone experience loss of DAPI staining following long PFA fixation?

We have some old mouse brains stored in 4% PFA for several years. DAPI and Hoechst stains are not working on this tissue, athough immunohistochemistry for GFAP is OK (after antigen retrieval)....

15 February 2024 6,600 3 View

What is the best MWCO of dialysis membrane for chitosan nanoparticles loaded with herbal extract?

I would like to run an invitro drug release experiment for chitosan nanoparticles (600nm) loaded with herbal extract using dialysis membrane method. However since its not easy to ascertain the...

06 February 2024 2,239 2 View

What are the reasons as to why Somaliland cannot be recognized as a State just like South Sudan was?

Somaliland is a self declared State running its own administration and having all the requirement to be a Sovereign State, but its not recognized by the UN. What is the AUs take on all this?...

18 January 2024 6,788 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View