I am trying to make dilutions from a commercially available plasmid stock of plasmid pBR 322. The buffer is Tris and EDTA pH 8. After I prepare the buffer, should I autoclave it for it to be DNAse free?

What type of water is good for standard buffer preparation for molecular biology research? I am Organic Chemist and beginning to work in the field of plasmids, so some guidance will be highly appreciated.

Thanks and kind regards

Ankita

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