Can anyone tell me the largest number of proteins directly extracted from soil ?

Can anyone tell me the largest number of proteins directly extracted from soil ? I am writing a paper about soil protein extraction and wanted to know what sort of yields other researchers were...

31 December 2019 7,930 2 View

We are looking for a Candicidin D standard ?

We are looking for a Candicidin D standard for HPLC and mass spectrometry. It is no longer made by the company listed on PubChem (alpha chemistry). Does anybody have any other suggestions?

31 December 2018 4,280 3 View

Are there specialised methods for extracting DNA from alkaline soils ?

I am conducting a metagenomic analysis of alkaline soil and I wondered if there were any specialised methods of DNA extraction given the alkaline nature of the soil.

07 August 2018 3,112 4 View

What is the maximum growth pH recorded for Streptomyces on solid media ?

I have been characterising a new strain of Streptomyces and was wanting to compare it to other similar alkaliphilic strains of Streptomyces. I have read of figures as high as 11.5 but the...

06 July 2018 1,383 8 View

Can you report a new bacteria on whole genome sequencing alone or do you have to have some polyphasic studies ?

I am being asked to provide polyphasic studies to name a new bacterial species, however, I have already provided the whole genomic sequence together with some comparitive genome analysis and some...

06 July 2018 1,916 0 View

What is the maximum pH level tolerated by Streptomyces coelicolor M145 ?

Are there any references to the maximum pH levels tolerated by strain S. coelicolor M145 ?

03 April 2018 2,449 4 View

What is the average difference in pH of agar before and after sterilisation ?

I had previously made a series of (modified SFM) agars at high alkaline pH 8----->13.6. I did not measure the pH of the resulting solidified agar because it just seemed a bit awkward and I...

02 March 2018 710 4 View

I was wondering if pH regulation in the region of amyloid deposition influences the development of Alzheimers ?

I was wondering if the pH of an environment influences the development of amyloid proteins ? Cellular control of pH is performed by porters/ antiporters. I have searched for these in human...

10 November 2017 9,237 4 View

What is the purpose of multiple copies of antibiotic resistance genes ?

We have sequenced a bacteria and found multiple copies of antibiotic resistance genes. The sequences of the copies are not identical. I was wondering if this diversity of copies is to increase the...

09 October 2017 6,310 13 View

How can you recover a protein sample which is tightly bound to a graphite column ?

I have been using activated carbon columns to clean up protein samples. Protein recovery has been very low. When I use a large amount of protein i.e. 5mg the recovery is only 0.5mg . I was...

09 October 2017 357 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View