Can anyone tell me the largest number of proteins directly extracted from soil ?

Can anyone tell me the largest number of proteins directly extracted from soil ? I am writing a paper about soil protein extraction and wanted to know what sort of yields other researchers were...

31 December 2019 7,930 2 View

We are looking for a Candicidin D standard ?

We are looking for a Candicidin D standard for HPLC and mass spectrometry. It is no longer made by the company listed on PubChem (alpha chemistry). Does anybody have any other suggestions?

31 December 2018 4,280 3 View

Are there specialised methods for extracting DNA from alkaline soils ?

I am conducting a metagenomic analysis of alkaline soil and I wondered if there were any specialised methods of DNA extraction given the alkaline nature of the soil.

07 August 2018 3,112 4 View

What is the maximum growth pH recorded for Streptomyces on solid media ?

I have been characterising a new strain of Streptomyces and was wanting to compare it to other similar alkaliphilic strains of Streptomyces. I have read of figures as high as 11.5 but the...

06 July 2018 1,383 8 View

Can you report a new bacteria on whole genome sequencing alone or do you have to have some polyphasic studies ?

I am being asked to provide polyphasic studies to name a new bacterial species, however, I have already provided the whole genomic sequence together with some comparitive genome analysis and some...

06 July 2018 1,916 0 View

What is the maximum pH level tolerated by Streptomyces coelicolor M145 ?

Are there any references to the maximum pH levels tolerated by strain S. coelicolor M145 ?

03 April 2018 2,449 4 View

What is the average difference in pH of agar before and after sterilisation ?

I had previously made a series of (modified SFM) agars at high alkaline pH 8----->13.6. I did not measure the pH of the resulting solidified agar because it just seemed a bit awkward and I...

02 March 2018 710 4 View

I was wondering if pH regulation in the region of amyloid deposition influences the development of Alzheimers ?

I was wondering if the pH of an environment influences the development of amyloid proteins ? Cellular control of pH is performed by porters/ antiporters. I have searched for these in human...

10 November 2017 9,237 4 View

What is the purpose of multiple copies of antibiotic resistance genes ?

We have sequenced a bacteria and found multiple copies of antibiotic resistance genes. The sequences of the copies are not identical. I was wondering if this diversity of copies is to increase the...

09 October 2017 6,310 13 View

Suggestion for optimimal protein extraction tips for graphite column ?

I am trying to optimise protein yields from a graphite column. The yields are in the order of 11% of the original protein. I have tried collecting the run through (contains protein) and using...

08 September 2017 6,692 2 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View