Can we patent a drug moiety arising out of natural product research?

Hi All We are involved in natural product drug research involving activity guided fractionation, structural characterisation and insilico science. I request your guidance in the patenting norms...

09 October 2018 9,839 0 View

How to interpret the nmr and mass spectral data?

Dear All Please guide me how to interpret the structure of phytoconstituent from crude extract of natural product sequentially Is there any online available tools to interpret the data

11 December 2016 3,727 3 View

How to optimize plant fraction for anti inflammatory potential?

Hi all I wish to optimise my antiinflammatory fraction of plant to successful lead against inflammation, After separation and optimisation insilico how to progress with and invivo evaluation of...

08 September 2016 499 3 View

How to assess the synergism/antagonism in plant extract?

Hi all I want to assess the synergism/additive/antagonism of individual fractions of plant extract, Is there any alternative methods other than isobolographic analysis? Kindly help me in this regard.

08 September 2016 807 3 View

Synthesis /Semisynthesis of natural product ? Design of synthesis procedure for natural product

Hi All I want to test my isolated phytoconstituent for some biological activity( i.e antidiabetic or anticancer).How to screen individual phytoconstituent or the synthetic analog is potent?. How...

03 April 2016 5,933 4 View

Can we study the anticancer activity of two phytoconstituents (in combination) to treat inflammation/cancer in cell lines/animal models ?

Hi All I am planning to study the molecular mechanism that link inflammation and cancer. I request you to advice me that whether two phytoconstituents(which share same biological property) can be...

02 March 2016 9,949 4 View

How to study the 3D QSAR modelling of phytoconstituents in Accelyrs Discovery studio?

Dear All We are doing pharmacophore modelling of our phytoconstituents from Brassica sps in Accelyrs Discovery studio. Kindly guide me in doing 3D QSAR modelling in Accelyrs discovery studio.

02 March 2016 5,238 0 View

How to quantify vitamins in plant extracts

01 February 2016 5,742 4 View

Quantification and identification of vitamin in plant extract How to quantify vitamins such as A D E K in plant extracts?

Your toughest technical questions will likely get answered within 48 hours on ResearchGate, the professional network for scientists.

07 August 2015 1,016 0 View

Can we patent a drug moiety arising out of natural product research?

Hi All We are involved in Natural Product Research.Can we patent a lead drug moiety arising out of Natural Product Research. Regds J Renuka

01 January 1970 9,786 15 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View

C-4 I cervical cancer cell line tissue culture protocol?

Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...

04 February 2021 10,018 5 View