Hello, i am currently studying on the property of different pH of chitosan on surface-modified PDMS. I found that pH 4.0- 6.0 was hydrophilic towards the surface-modified PDMS... but pH 5.5 showed an extreme hydrophobic (>90 degree). any reason why?
Quite possibly, pH=5.5 is its isoelectric point.
Hi I have tried to measure the mobility of a protein of interest by the FRAP assay. The protein was fused with GFP. I overexpressed the fusion protein in Hela cells. However, when the area of the...
14 December 2020 6,567 1 View
My oleate-capped NaCeF4:Gd,Tb NPs have been synthesized via the thermal decomposition method. 1mmol of NPs has been dispersed in 20 ml of cyclohexane. I have tried dissolving 1 ml of NP in...
01 December 2020 4,713 2 View
Hi, When checking my designed primers for specificity, what is the difference if I run BLASTn for each primer individually versus together (ie. forward primer separated by 20 "N" then reverse...
03 November 2020 1,421 4 View
Hi all, Recently I encountered some irregular black/grey patches inside my cell culture flask (see attached image, x400 magnification). They are not motile, not floating, do not stick to cells...
08 July 2020 7,921 3 View
Hi all, I am facing a problem to trypsinize and combine/move the transfected cells into new well . I was planning to pool the transfected cells into a new well to ensure better cell-cell...
21 December 2019 9,692 5 View
Hi all, I am facing a problem to generate a stable cell line after successful transfection. I added puromycin to kill the untransfected cells, however, eventually all my transfected cells are...
19 September 2019 2,970 2 View
Hi, Is there any paper which reported the co-transfection of miRNA mimics and inhibitors? Thank you.
17 June 2019 1,087 3 View
Dear all, I have a miRNA primer sequence left by someone else and I would like to know about the sequence of this primer. Is there any method I can use to sequence and know the sequence of this...
14 June 2019 5,304 6 View
TO The Editor: You credit me falsely with written Research articles that I, Angeline butler, have not written. Please correct your communications to your population. I have not written any...
29 April 2019 290 2 View
Hi all, I am planning for some transfection work involving a mammalian cancer cell line. Can I know the transfection kit from Qiagen or ThermoFisher lipofectamine is a better option? Do you...
23 March 2019 9,831 3 View
When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...
03 March 2021 4,892 5 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
[Synthesizing Cu nanoparticles without using chemical reductants]
01 March 2021 8,359 1 View
Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...
01 March 2021 6,187 2 View
Incompletely cross-linked pdms are difficult to remove.
01 March 2021 798 3 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...
27 February 2021 947 7 View
Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...
26 February 2021 5,525 3 View