We are looking for a reliable source of PGP 9.5 antibody to stain human and mouse skin biopsy samples. We tried several sources and not satisfied. The one that worked well was serotec Rabbit polyclonal but this has been replaced with a mouse monoclonal. We are not getting good staining. Our method is primary overnight @ 4 deg C without antigen retrieval and secondary is Vector ABC SG kit. We appreciate your help, thoughts and suggestions.
I was once using a PGP9.5 antibody from Biomol International and it gave beautiful results. This company was bought by Enzo. I'm pretty sure the antibody is now on this webpage: http://www.enzolifesciences.com/ADI-905-520/pgp9.5-polyclonal-antibody/
I also added the publication in which it was used by us.
Good luck.
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Thanks Dr.Auclair
I didn't get the publication, I will check it in Pubmed. Did you use it for measuring intraepidermal nerve fiber density measurements? human or rodents?
Hi,
You can check an antibody database at Labome.com. For PGP9.5 antibodies, you can check this link: http://www.labome.com/review/gene/human/UCHL1-antibody.html. Bio-Rad offers one mouse monoclonal anti-human PGP9.5 antibody (31A3) and mouse monoclonal anti-mouse PGP9.5 (13C4) antibody for IHC. Also, Abcam and EMD Millipore have guinea pigs polyclonal anti-mouse antibodies suitable for IHC (reference: Avula et al. Histochem Cell Biol 2013 and McCoy et al. Am J Physiol Endocrinol Metab 2013). In addition, Invitrogen has rabbit polyclonal anti-mouse antibody (38-1000) (reference: Jiang et al., Biol Reprod 2015) and Dako provides rabbit polyclonal anti-human antibody (Z5116) (reference: Ramos-Vara and Miller Vet Pathol 2007). I hope this comment is helpful.
Hello everyone, I have the same query as Krish. I am looking for a reliable PGP9.5 primary antibody to stain the intraepidermal fibres in mouse hindpaw skin. The one I used earlier and which worked well was from Abd serotec Rabbit polyclonal but this has been replaced with a mouse monoclonal (which doesnt work). All suggestions would be welcome. Thank you!
Hi Puneet
We found that the Thermo Fischer Scientific rabbit polyclonal PA5-29012 works very well with mouse skin biopsy, so we stocked it up. Check this out. It is bit expensive though, it is reasonable if you use 96-well plate method.
Hi Krish
Thank you for the suggestion! We do the immunohistochemistry with the frozen fixed sections of mouse hind paw skin. So how would the 96 well plate method go along with this. Any suggestion. Thanks!
Puneet, if you have not used 96 well method, it takes time to set it up, you have go through many trials and errors, it may be better to use the method that you are currently using and that works well.
Puneet
I am working on diabetic neuropathy, are you, by chance, working on the same area or some other area? thanks.
Hi Krish
I am actually working on the spared nerve injury and how this effects the intraepidermal fibres and the terminal schwann cells.
Hi Krish
Just a quick question. Do you also stain the glial cells (like schwann cells) in the mouse skin biopsy. We have one from dako made in rabbit but since PGP9.5 (Thermo fischer) is also made in rabbit, so we cannot mix them togethere. Any idea? Thanks!
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