Let me know whether the DOI issued by the research gate will be accepted by the anna university?

As per the rules and regulation DOI is needed and paper has to be opened in online by the anna university rules let me know whether the published article in some journal and the DOI issued by...

27 November 2020 7,540 2 View

Can anyone suggest a way to overcome recombination when amplifying a pcDNA3 plasmid containing human SCN9A CDS insert?

I need to amplify a pcDNA3 plasmid containing human SCN9A CDS insert (pcDNA3-SCN9A construct). The CDS is around 6 kb, and the total construct length is approximately 11.2 kb, which is a bit...

07 June 2020 2,134 2 View

Does anyone have the Grass Instruments/AstroMed S88X Stimulator Manual?

Hello Everyone, Does anyone by chance have a Grass Instruments/Astro Med S88X stimulator manual? (http://www.neurolabparts.com/stimulators/99-grass-astro-med-stimulators-model-s88x.html). I...

17 December 2019 1,447 1 View

How to develop MCF7 mouse xenografts?

Hello everyone, I have two questions, please shed some light on this. I am new to in vivo studies and some may find my questions are trivial. TIA. My first question is, I wanted to develop the...

13 October 2019 1,208 2 View

Looking for opensource tool for ReaxFF simulation. If there something other than paid software ADF please reply ?

Looking for opensource tool for ReaxFF simulation. If there is something other than paid software ADF please reply ?

24 January 2018 6,596 5 View

Is it necessary to synthesis second strand cDNA to amplify the gene of interest?

Dear All, I have synthesised cDNA first strand using SuperScript III (Invitrogen) and would like to amplify my gene of interest for overexpression studies? Is it necessary to synthesis second...

08 November 2017 5,895 11 View

How to clone a 5 kb insert to a vector successfully?

Hello, everyone. I'm trying to subclone a 5 kb gene to a vector (approx. 5 kb). Initially, I designed the primers which have restriction enzymes overhang (XhoI and XbaI). I amplified my gene using...

27 August 2017 4,722 4 View

Does accumulation of Enzyme- substrate complex possible inside a cell? Is there any experimental evidence which confirms their accumulation?

I am a systems biologist trying to study cellular cross talk. Some computational papers have mentioned about the effect of enzyme substrate complex on the cellular cross talk. So, my question is...

18 December 2016 7,172 3 View

No title

25 July 2016 7,622 1 View

PGP9.5 antibody for human and mouse skin biopsy samples?

We are looking for a reliable source of PGP 9.5 antibody to stain human and mouse skin biopsy samples. We tried several sources and not satisfied. The one that worked well was serotec Rabbit...

08 June 2016 5,874 12 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

Can bio-decomposer be a successful alternative to reduce residue burning?

To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...

16 February 2021 7,068 24 View

Reaction condition to convert NH2 to COOH?

I need to couple Dopamine by NH2 end to silica nanoparticles coated by PEG-NH2, but first i need to convert the PEG-NH2 to COOH by using succinic anhydrous ... can you suggest a reaction condition ?

13 February 2021 2,768 4 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

What is the type or grade of ethanol used in SOL-GEL?

I'm preparing materials that will be use in SOL-GEL to produce silica gel, I will use Tetraethyl orthosilicate (TEOS) as precursor, and ethanol as solvent , but when I search to buy ethanol, the...

01 February 2021 5,153 4 View

What is the most efficient way to generate amorphous silica in LAMMPS?

I would like to generate multiple large samples of amorphous silica in LAMMPS. Currently I am generating it by beginning with a lattice of silicon and oxygen atoms and using a melting and...

28 January 2021 6,128 4 View