Has anyone attempted to transfect ES cells with Cas9 RNP/sgRNA/ssDNA using Lipofectamine?

Arun Pandian Chandrasekaran @Arun-Pandian-Chandrasekaran

26 March 2023 0 4K Report

I am attempting to insert a reporter gene into human embryonic stem cells using Lipofectamine-based reagents such as Lipofectamine 3000, CRISPRMAX, and RNAiMAX. Has anyone had success with these reagents? If so, could you recommend the appropriate Cas9 RNP/sgRNA/ssDNA ratio to use?

Many thanks in advance,

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