Which preparative resin or gel would be ideal to separate a peptide from the mixture of amino acids, amino acid derivatives, vitamins and organic acids?
Please suggest.
Antierum titer determination is usually done by its serial dilution and then taking the lowest positive value in comparison to blank. People take minimum twice or thrice of blank as positive...
31 December 2014 3,537 2 View
In antibody titer determination, we generally compare the signal to noise ration. I guess 2:1 or 3:1 is taken as a positive titer. Is this also referred to as good titer? Are there any references...
10 November 2014 6,291 4 View
Antibodies were raised against the whole cell extract.
03 April 2014 1,544 0 View
To remove any doubt all other aspects were checked such as blocking buffer, plates, etc...
03 April 2014 2,415 8 View
My sample contains a peptide which is acidic in nature of very very low concentration and with not known sequence. How can I isolate it and identify it. I already used SEC and IOn exchange to...
01 February 2012 1,757 8 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...
28 February 2021 6,477 1 View
Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...
24 February 2021 2,602 1 View
Looking for method of extracting and analyzing vitamin D in foods by HPLC
24 February 2021 7,524 7 View
What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?
23 February 2021 1,832 4 View
My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...
21 February 2021 5,656 3 View
This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...
21 February 2021 546 3 View
I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...
20 February 2021 5,820 4 View