Antibodies were raised against the whole cell extract.
Antierum titer determination is usually done by its serial dilution and then taking the lowest positive value in comparison to blank. People take minimum twice or thrice of blank as positive...
31 December 2014 3,537 2 View
In antibody titer determination, we generally compare the signal to noise ration. I guess 2:1 or 3:1 is taken as a positive titer. Is this also referred to as good titer? Are there any references...
10 November 2014 6,291 4 View
To remove any doubt all other aspects were checked such as blocking buffer, plates, etc...
03 April 2014 2,415 8 View
My sample contains a peptide which is acidic in nature of very very low concentration and with not known sequence. How can I isolate it and identify it. I already used SEC and IOn exchange to...
01 February 2012 1,757 8 View
Which preparative resin or gel would be ideal to separate a peptide from the mixture of amino acids, amino acid derivatives, vitamins and organic acids? Please suggest.
01 February 2012 9,504 0 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
02 March 2021 10,003 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View
