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Questions related from Zulfaquar Ahmad Arfi
Antierum titer determination is usually done by its serial dilution and then taking the lowest positive value in comparison to blank. People take minimum twice or thrice of blank as positive...
01 January 2015 3,571 2 View
In antibody titer determination, we generally compare the signal to noise ration. I guess 2:1 or 3:1 is taken as a positive titer. Is this also referred to as good titer? Are there any references...
11 November 2014 6,327 4 View
To remove any doubt all other aspects were checked such as blocking buffer, plates, etc...
04 April 2014 2,455 8 View
Antibodies were raised against the whole cell extract.
04 April 2014 1,577 0 View
My sample contains a peptide which is acidic in nature of very very low concentration and with not known sequence. How can I isolate it and identify it. I already used SEC and IOn exchange to...
02 February 2012 1,797 8 View
Which preparative resin or gel would be ideal to separate a peptide from the mixture of amino acids, amino acid derivatives, vitamins and organic acids? Please suggest.
02 February 2012 9,576 0 View