Amount of metal loaded?

how to calculate the amount of loaded metal nanoparticles on graphene support?

07 January 2021 7,277 2 View

Is their any relation between modic changes of vertebra and diabetes?

I need to know if their is any relation between modic changes in the vertebra and diabetes. And if any one aware of any paper in literature regarding that

23 March 2019 8,331 4 View

What are the real indication of OLIF a Nd DLIF in spine surgery?

08 June 2016 9,438 3 View

How can i plot Cp or Nu around a circle in CFD-Post or fluent?

Hello guys, i have been working on this problem for quite some time now, i was able to plot Nu vs theta using poly lines,expressions and variables however the plot does not start from 0 degrees...

11 February 2016 195 6 View

How can I recover protein sample from RIPA buffer?

16 October 2015 798 13 View

Has anyone used centrifugal concentrator tubes?

I have transfected L929 cells with polio virus in T-75 flask. I left it for 48 hours and now I collected the media which is 15ml DMEM. I want the best way to isolate the viral RNA from this media....

06 October 2013 6,929 4 View

Quality of the training environment

Quality of the training environment is new term, Includes all appliances, tools and sports fields, and the impact on the psychological athlete. who can give helpful?

01 January 1970 6,653 1 View

How i can separate bacterial cells from LB broth to further inoculate it in MSM media ?

01 January 1970 191 4 View

Radical Distribution Function using AMBER

I am trying to run a Radial Distribution Function (RDF) trajectory analysis between water (WAT) hydrogen atoms and ligand (UNN) nitrogen atoms using the production trajectory (prod1.crd) and the...

01 January 1970 7,434 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

Why are Sanger Sequencing reads interrupted in some edited clones?

I have a Crispr/Cas9 edited bulk population and I can briefly check the indel ratios in the bulk population using Sanger sequencing. After cloning single cells, I wanted to check the indel types...

18 February 2021 8,198 6 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View