I'm developing novel cancer drug utilizing Patient-derived xenograft (PDX) mouse.
Currently, I am obtaining human tumor tissue samples from domestic hospitals for my research. But I countered big problem.
After I transplant tissue samples on mice subcutaneously, the human tumor generates abscess within 1 months. Do you have any idea why the tumor is not stable and is being damaged?
I guess that, because of BALB/c-nude mouse has B cell and innate immune cells, although these cells do not have full-function, the transplanted tumor can be attacked.
However, when I have used NSG mouse instead of BALB/c-nude mouse, the tumor did not grow within 6 months and transplanted mice were dead.
How often do such incidents take place?
I appreciate your interest. Thank you. :)
I have not done this myself, so this may be an incorrect answer, but I think it's worth mentioning - abscess/pus sounds like a bacterial infection to me. How certain are you that your sterile technique (both for harvesting and transporting the human samples, AND performing the xenograft surgery) is good enough, given that these are immunodeficient mice? Does your surgery protocol include treating the mice with antibiotics for a period of time after surgery to eliminate any bacteria that were accidentally introduced? If you don't already do this, I think it would be worth trialling antibiotics to see if they help.
Laura Leighton I appreciate your answer!
In fact, the problem is last so long time.
I already received tissues more than 10, but most of these cases generate abscess or pus.
And in my lab, many experiments are conducted and followed by sterilization daily.
So I consider that this trouble is not triggered by contamination, but the immune system of nude mice.
If you have any other discussable issue or opinion with this circumstance, please answer again.
Thank you for your kindness!
Could be human immune cells from the tumour samples attacking the mouse tissue.
Robert Adolf Brinzer Thank you for your opinion. I know that the life span of human white blood cells are 2 to 15 days, including innate immune cells. However, in our studies, the transplanted tumor were maintained on the mouse's shoulder for 4~6 months. Also tumor tissues do not develop abscesses within a few weeks but take more than 1 months.
So, in my thought, the immune cells couldn't affect on human tumor tissues within their life span.
Do you have any further ideas? Please share with me :)
Abhijeet Parkhe
I regret to inform you that I have not yet found the answer.However I just try to incubate the part of human tumor tissue on cell plates, like 24 wells plate for checking proliferative ability.
After 1~2 weeks, I could observe the increased branch of differentiated cell, like stem cells, on the bottom of culture plate. This situation means the tissue has proliferative ability.
Then, in my opinion, because of inappropriate nutritional environment, in mouse body, the tissue couldn't survive and went through apoptotic processes.
Do you have any clue or opinion, please share with me. Thank you :)
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