PCR reaction problem ?

07 July 2018 0 8K Report

I prepared PCR rxn with phire DNA polymerase and my amplicon is 1.6 kb long. As you can see in gel photo the amplicon is located at 1.5 kb. I was expecting it to locate a bit above. I will use it for cloning, I purified the band from gel and I will ligate. Do you think something is wrong in PCR or my amplicon size is correct but something was wrong at soidification of that part of gel?

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