Furkan Oflaz

6 Questions 11 Answers 0 Followers

Questions related from Furkan Oflaz

How can I introduce restriction site in the middle of my insert?

Does anyone know any kit free protocol for adding restriction site in the middle of insert ?

09 September 2018 3,076 0 View

Sequence of PTZ57R/T plasmid ?

Does anyone has the sequence of PTZ57R/T plasmid? I am planning to send my constructs to sequencing that is why I need the map of PTZ57R/T plasmid. Anyone ?

08 August 2018 8,326 0 View

PCR reaction problem ?

I prepared PCR rxn with phire DNA polymerase and my amplicon is 1.6 kb long. As you can see in gel photo the amplicon is located at 1.5 kb. I was expecting it to locate a bit above. I will use it...

07 July 2018 7,544 0 View

TA cloning problem. Is it possible two or more inserts to ligate with a plasmid in TA clonig ?

I did TA cloning with instaclone PCR cloning kit and I got some weird results. I picked 12 white colonies, isolated them and cut with a single cutter enzyme BamHI (I cut with BamHI to linearize...

07 July 2018 9,453 4 View

Protocol for A-Tailing with Taq Polymerase ?

I am planning to clone 1.3-1.5 kb inserts to plasmid by using TA cloning but I am worrying about error rate of Taq polymerase, that is why firstly I am plannig to do PCR with high fidelity enzyme...

07 July 2018 5,932 0 View

Why I got unexpected larger bands after digestion of my vector (with insert) ?

For my cloning experiment, I first ligated my insert (1.5 kb) with linearized vector (2.9 kb ) that is provided with instaclone PCR cloning kit. After plating, I randomly selected 5 colonies from...

07 July 2018 2,435 10 View