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Questions related from Furkan Oflaz
Does anyone know any kit free protocol for adding restriction site in the middle of insert ?
09 September 2018 3,075 0 View
Does anyone has the sequence of PTZ57R/T plasmid? I am planning to send my constructs to sequencing that is why I need the map of PTZ57R/T plasmid. Anyone ?
08 August 2018 8,324 0 View
I prepared PCR rxn with phire DNA polymerase and my amplicon is 1.6 kb long. As you can see in gel photo the amplicon is located at 1.5 kb. I was expecting it to locate a bit above. I will use it...
07 July 2018 7,543 0 View
I did TA cloning with instaclone PCR cloning kit and I got some weird results. I picked 12 white colonies, isolated them and cut with a single cutter enzyme BamHI (I cut with BamHI to linearize...
07 July 2018 9,452 4 View
I am planning to clone 1.3-1.5 kb inserts to plasmid by using TA cloning but I am worrying about error rate of Taq polymerase, that is why firstly I am plannig to do PCR with high fidelity enzyme...
07 July 2018 5,931 0 View
For my cloning experiment, I first ligated my insert (1.5 kb) with linearized vector (2.9 kb ) that is provided with instaclone PCR cloning kit. After plating, I randomly selected 5 colonies from...
07 July 2018 2,433 10 View