Protocol for A-Tailing with Taq Polymerase ?

07 July 2018 0 6K Report

I am planning to clone 1.3-1.5 kb inserts to plasmid by using TA cloning but I am worrying about error rate of Taq polymerase, that is why firstly I am plannig to do PCR with high fidelity enzyme (generates blunt end) than add 3'A with Taq polymerase (sigma aldrich).

Any tried protocol suggestion?

Regards.

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