Commonly employed tests for detection of Streptococcus pyogenes include bacitracin sensitivity, PYR test and latex agglutination. But we can't completely rely on these tests as the results are not reproducible at times. With some isolates, I am facing the same problem, ie, when I repeat the tests for a second time the results are different especially with latex agglutination.How can I confirm the isolates as Streptococcus pyogenes. can we use spy1258 and dnaseB pcr for this purpose? how specific is these two pcr for detection of Streptococcus pyogenes. Please help me
You must design a primer by primer 3 or generunner or pickprimer in ncbi using streptococcus pyogenes genome and then recheck primers with primer-blast in pubmed
Hello Tintu.
RAPD cld be the solution for your problem, though its meant to be for typing.
You wd hv already seen these 2 attached papers.
All the best.
Please find enclosed another paper about molecular detection of streptococcus pyogenes using speB detection.
Best wishes
Pierre
qPCR with SpeB primer sets wd be a gd option (as per attached paper of Dr. Pierre).
Thank you all.
I did spy1258 and dnaseB PCR for isolates of S.pyogenes (PYR positive, bacitracin zone above 15mm and latex agglutination positive for group A) along with streptococci of other groups (B,C,D,F,G) and those with non reproducible results.
spy1258 gene PCR correctly identified all S.pyogenes isolates and was negative for others.
dnaseB pcr is not specific for S.pyogenes as it gave positive results with other groups also.
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