Tintu Abraham

6 Questions 5 Answers 0 Followers

Questions related from Tintu Abraham

How much copy number of bacterial DNA to be present in the throat swab of healthy children to confirm him/her as an asymptomatic carrier?

I am using spy gene qPCR for detection of asymptomatic Group A streptococcal carriers. A Ct value of less than or equal to 37 is taken as positive. Is this the right way? Is there any specific...

11 November 2017 2,661 0 View

PCR detection of Streptococcus pyogenes?

Commonly employed tests for detection of Streptococcus pyogenes include bacitracin sensitivity, PYR test and latex agglutination. But we can't completely rely on these tests as the results are not...

08 August 2014 5,616 5 View

What is the best method for DNA extraction of streptococcus?

In order to extract DNA from Streptococcus from pure culture which method is recommended. I found many manual extraction methods, some recommend use of mutanolysin/ lysostaphin for the better...

07 July 2014 4,083 8 View

Which target sequence can be used for specific identification of Streptococcus pyogenes using PCR?

When using the latex agglutination test (plasmatec) for streptococcal grouping, sometimes for a single isolate I am getting two or three groups. The Biomerieux grouping kit is very costly. What...

03 March 2014 338 3 View

Which target sequence can be used for specific identification of Streptococcus pyogenes using PCR?

In order to run a PCR specific for Streptococcus pyogenes, which target sequences should I use?

03 March 2014 5,514 1 View

Can anyone provide the methodology for emm typing for Streptococci?

I am working on the molecular epidemiology of beta haemolytic Streptococcus. Although I have the CDC guidelines for emm typing, I am confused in procedure especially after PCR step. Can anyone...

01 January 2014 2,090 4 View