PCR data analysis softwares

More Azeem Mehmood Butt's questions See All

Is there any free geographic information system (GIS) software to map epidemiology of a disease and its features on a country map?

Dear all Could anyone please tell me how to integrate epidemiological data with region? I am looking for a way/tool to show epidemiology of a disease and its features on a country map? For...

08 September 2016 8,531 7 View

What is best way to correlate real time PCR values with biochemical features?

Hello everyone I would like to ask question about situations where gene expression values are used to correlate with clinical features of samples under investigation. For example, in an...

31 December 2014 4,068 5 View

Has anyone used the spike-in for circulating microRNA?

I would like to know if there is anyone who has worked specifically with QIAGEN cel-miR 39 spike-in using the QIAGEN miRneasy mini kit.

04 May 2014 9,484 3 View

Why am I getting aqueous phase contamination with Trizol?

I am using the QIAGEN miRNeasy kit for total RNA isolation from tissue and serum samples as per manual instructions. However, I am facing issues with the phenol contamination and the 260/230...

04 May 2014 311 7 View

Can anyone advise me on codon usage analysis, ENC vs GC3 values?

In codon usage studies, it is observed that a correlation between ENC and GC3 values is plotted as a curve following Wright's, 1990 method and hypothesis. However, what should be the case when a...

11 December 2013 784 1 View

Rights of students enrolled in Universities and Research Institutes?

I would like to have your opinions on rights of students when they are enrolled in different degree programs considering following case as an example: 1. A student is studying for his/her higher...

08 September 2013 9,303 9 View

Why is the polynomial trend line inverted?

I am trying to perform the correspondence analysis between the set of values of GC3 and ENC, given in the attached Microsoft Excel sheet. The problem is that I have applied trend line (polynomial)...

07 August 2013 6,138 2 View

Could anyone please tell me if is there any tool/server available for miRNA target prediction in user specified genomes?

There are several target prediction tools but they are limited in terms of options that we can select humans, mouse etc genomes. What if the genome sequence of any other organism, which is not...

06 July 2013 9,981 2 View

COX protein isolation from Blood/serum

Hello everyone Could anyone please tell me is it possible to isolate COX protein from serum or whole blood samples for western blotting?

02 March 2013 8,469 2 View

Annotation of viral genomes

Please suggest a reliable free software/server for annotation of newly sequenced viral genomes

01 February 2013 2,322 7 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Sven Dittmann

Hello Azeem,

look here: http://www.gene-quantification.info/ and then Cooperating Institutions & Laboratories I think GenEx software or qBASEPLUS maybe works.

Jo Vandesompele

qBase was originally developed under my supervision at UGent. It is now commercialized by UGent spin-off Biogazelle. The original paper has more than 600 citations.

http://www.qbaseplus.com

You may also consider LinRegPCR from Jan Ruijter. Now reads and exports RDML format. LinRegPCR RDML export can be directly imported in qbasePLUS.

http://www.hartfaalcentrum.nl/index.php?main=files&sub=LinRegPCR