How to remove compound ( Europium complex ) from paraffin oil?

How can I remove my compound from paraffin oil? Mistakenly my compound fell in an oil. Compound = Europium complex

22 February 2021 5,667 2 View

Regarding Chemkin.. no reaction taking place after simulation. What to do?

I am simulating cyclohexanone oxidation from a paper. I got the input files from supplementary data. But when I am simulating, and generating Excel file at different temperatures the reactant...

02 October 2020 6,591 4 View

Can someone provide the list of cell lines available at NCCS, Pune, India?

I need to purchase a few cell lines but I am not sure whether those are available with them or not.

31 December 2018 3,313 7 View

Can someone suggest some brain endothelial cell lines?

Preferably Biosafety level 1. Also, if you can tell from where we can procure them in India.

11 December 2018 8,356 6 View

Can someone suggest a method for revival of THP-1 cell line?

Cells are healthy when used routinely but once freeze downs are made (FBS + 10%DMSO) and stored in -80 C their revival is quite difficult and majority cells are dead. I am using RPMI-1640 media...

15 June 2018 5,833 3 View

Is there a standard method for extraction of essential oil of aromatic plant leaves using solar distillation technique?

28 September 2017 4,608 3 View

How can one ensure ligation of a 300bp fragment into 5369bp host?

18 October 2014 5,087 15 View

How to use a Western Blotting specific antibody for IHC (for PFA fixed & paraffin Embedded tissue)?

I have an antibody in my lab specefic for western blotting only, but I need to detect same antigen in a PFA fixed tissue. I tried to retrieve epitopes using Citrate buffer, HCL treatment &...

16 October 2014 5,595 7 View

Can anyone comment with examples on the idea that disasters set the development clock back to several decades?

Recently, the increasing number of disasters has shook the world. Be it the cloud burst in Uttrakhand, India or cyclone Katrina in USA or Typhoon Haiyan in Phillipins. What is the exact amount of...

30 November 2013 4,686 4 View

Exchange of fatty acid standard for GC-MS

Hi, I have a request to make here. We have purchased a fatty acid standard to carry out some GC-MS experiments, but we never got to conduct them. The standard is...

01 January 1970 1,562 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View