Regarding Chemkin.. no reaction taking place after simulation. What to do?

I am simulating cyclohexanone oxidation from a paper. I got the input files from supplementary data. But when I am simulating, and generating Excel file at different temperatures the reactant...

02 October 2020 6,591 4 View

Can someone provide the list of cell lines available at NCCS, Pune, India?

I need to purchase a few cell lines but I am not sure whether those are available with them or not.

31 December 2018 3,313 7 View

Can someone suggest some brain endothelial cell lines?

Preferably Biosafety level 1. Also, if you can tell from where we can procure them in India.

11 December 2018 8,356 6 View

Can someone suggest a method for revival of THP-1 cell line?

Cells are healthy when used routinely but once freeze downs are made (FBS + 10%DMSO) and stored in -80 C their revival is quite difficult and majority cells are dead. I am using RPMI-1640 media...

15 June 2018 5,833 3 View

Is there a standard method for extraction of essential oil of aromatic plant leaves using solar distillation technique?

28 September 2017 4,608 3 View

PCR amplification of nearly 200bp fragment from bisulfite converted DNA not working.

I am trying to amplify nearly 200bp segment of DNA. Changed primer concentrations, template concentration, primer stock everything, still no amplification. Seeking help.

17 July 2016 1,417 6 View

How can one ensure ligation of a 300bp fragment into 5369bp host?

18 October 2014 5,087 15 View

How to use a Western Blotting specific antibody for IHC (for PFA fixed & paraffin Embedded tissue)?

I have an antibody in my lab specefic for western blotting only, but I need to detect same antigen in a PFA fixed tissue. I tried to retrieve epitopes using Citrate buffer, HCL treatment &...

16 October 2014 5,595 7 View

Can anyone comment with examples on the idea that disasters set the development clock back to several decades?

Recently, the increasing number of disasters has shook the world. Be it the cloud burst in Uttrakhand, India or cyclone Katrina in USA or Typhoon Haiyan in Phillipins. What is the exact amount of...

30 November 2013 4,686 4 View

Exchange of fatty acid standard for GC-MS

Hi, I have a request to make here. We have purchased a fatty acid standard to carry out some GC-MS experiments, but we never got to conduct them. The standard is...

01 January 1970 1,562 1 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

When I mix oil and NaOH solution, Why solid layer in the surface is formed?

During my trial for lubricant oil re-refinery process, I use NaOH as coagulant agent. After overnight of gravity settling, a solid layer in the surface was form. what product is that? since the...

22 February 2021 2,116 4 View

The type of filter paper used to filter an epoxidized oil (the mixture is an epoxidized oil with magnesium sulfate) ?

I have a mixture of epoxidized oil and magnesium sulfate wich type of filtre paper should i use

21 February 2021 3,944 3 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View

How can we study the adsorption efficiency of microgel at the oil-water interface?

I am studing the mircogel at oil-water interface. I want to know the adsorb amount of microgel at the oil water interface. How we can determine this amount?. I will highly appreciate your...

19 February 2021 2,787 1 View

Does anyone know, can i do cryosectioning of whole aorta after oil red O staining and use the cryosection parts for further analysis?

I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...

16 February 2021 5,876 2 View

Can any one explain why the ethyl ester Biodiesel (FAEE) has higher than that of the metyl ester FAME- both of them are derived from the same oil?

Biodiesels derived from waste vegetable oil transesterification with Ethanol (FAEE) show higher peroxide values than those derived by transesterification with methanol (FAME) prepared under the...

13 February 2021 193 6 View

How to do immunofluorescence staining of the microtome sections of paraffin-embedded tissue ?

I plan to do immunofluorescence staining of microtome sections of paraffin-embedded metastatic tumor tissue with two or more than two antibodies with different emission spectra. Should I follow...

10 February 2021 3,113 3 View