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I want to deplete a gene X in yeast using the TetOff and AID system at the same time. I transformed two strains in TetO-X and X-AID and I am now asked to do backcross to get TetO-X-AID.So, I am...
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The medium is YPAD, and it is probably contaminated with bacteria. I suppose this kind must synthesize some pH sensitive molecules as It is reacting with bleach but I can't find any information on...
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