Does anyone know if there is an inhibitor cocktail that will block all (or at least most) serine/threonine phophatase activity in cells? Ideally I'd like to be able to pretreat cells to increase the pool of phospho-proteins within the cell then lyse the cells and preform pull-down assays to see if my phopho-protein of interest shows differential binding to a known substrate.
Steven
You may need to use a few compounds to block phosphatases - refer to the link below
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/protease-phosphatase-inhibitors.html
Sodium fluoride is a phosphoseryl and phosphothreonyl phosphatase inhibitor that our lab regularly uses. We also generally use it with sodium orthovanadate to inhibit phosphotyrosyl phosphatases as well.
Check out Halt™ Phosphatase Inhibitor Cocktail Catalog number: 78420 from thermofisher
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