P3 buffer (Lonza nucleofactor) alternative with lower cytotoxicity for nTreg transfection?

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Vigneshwaran Venkatesan

Dear Nadja Nicola Ilkenhans ,

I don't think the P3 solution is toxic. Kindly optimize the nucleofection codes. That's the key. Consider using CA137.

Nadja Nicola Ilkenhans

Thank you very much for recommending the program! have you used it before for murine T cells or specifically Tregs? I did not consider this program as I thought it's primarily used for human stem cells.