Vigneshwaran Venkatesan

2 Questions 9 Answers 0 Followers

Questions related from Vigneshwaran Venkatesan

Targeted knock out of a gene by inserting a fluorescent protein sequences in the exon of the desired gene ?

Hi everyone, Thanks for your time. I am planning to knockout a gene by targeted insertion of RFP into the exon 1 by CRISPR cas mediated HDR. Since insertion of the promoter...

07 March 2019 9,518 1 View

IPSC - E8 - Can i aliquot E8 supplement and store it in -20 and adding to the Basal E8 medium when required?

In E8 medium user guide, it is mentioned that the Basal media with supplement can be used only for 2 weeks. But the basal media without supplement have a shelf life of 8 months. Can i aliquot the...

11 April 2018 7,493 1 View