Hi, every one,
if I want to prepare a tumor cell line expressing OVA to carried out antigen presenting experiment. which fragment I should chose? The full length protein or the fragment of SIINFEKL? Thank you very much.c
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,667 5 View
The laser confocal system of OLS5100 (Olympus) can reach a lateral resolution of 120 nm, that is far beyond the diffraction limitation. Though the numerical aperture of its system is 0.95, the...
15 May 2024 1,458 3 View
As known, the spin exchange coupled Mn4+ leads to a narrowd singal, at ca. g=2.006 with the line width about 500 gauss. Why the birnessite MnO2 dispalys such wide spectrum? Is any other exchange...
23 April 2024 6,349 3 View
The silver wire has been used as a reference in an organic electrolyte in many artcles. What is the standard electrode potential of the silver wire reference electrode and how to calibrate its...
14 April 2024 8,051 3 View
I'm just looking for iso 29134 pdf, does anybody have any idea where I can download it for free?
08 April 2024 6,507 0 View
Edge computing is a decentralized computing infrastructure that brings computing power closer to the data source, reducing latency and improving efficiency. Traditionally, computing tasks were...
19 March 2024 1,229 0 View
How to sterilize a polymer grafted with a photoinitiator before cell culture?
23 January 2024 9,548 2 View
I want to use carboxymethyl cellulose for an EDC/NHS reaction to attach amine-functionalized small molecules. Initially, I used an EDC:NHS ratio of 1:1, but the grafting efficiency was low. Later,...
11 January 2024 6,871 1 View
When the LSV test is conducted with the prepared material, the image of linear decline will appear at last, but this problem will not occur when I change to other materials (such as new foam...
04 January 2024 9,718 5 View
I have no issues with my plastic hinge settings, and it converges well in V14. However, when opening the file in V20, it fails to converge. How should I address this? I have set the hinge...
19 December 2023 4,910 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,822 3 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,841 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,104 4 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,216 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 639 2 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,281 2 View