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Questions related from Fengshuang Yi
We know that when we use prokaryotic cells such as E. coli to express proteins, we often transform the E. coli with a plasmid containing the target gene, then screen for positive clones through...
01 March 2025 1,144 4 View
Dear all, Drug resistance mutation EGFR T790M occurs after treating with first line EGFR-TKIs to lung cancer patients with activating mutation like L858R. I wonder that in the most of cases, two...
27 July 2022 1,540 0 View
Hi, every one, if I want to prepare a tumor cell line expressing OVA to carried out antigen presenting experiment. which fragment I should chose? The full length protein or the fragment of...
27 May 2022 7,935 0 View
Recently, we have done the macrophage antigen lpresenting experiment by co-culture of macrophages pre-loaded with OVA and CD4+/CD8+ T cells isolated from OT-II/OT-I mouse spleen. Using CFSE to...
11 May 2022 1,781 6 View
Dear all, I have been working on antigen presenting experiments in cancer pathogenesis, recently, I am just wondering that is there any tumor-associated antigen that can be chosen to stimulate T...
23 November 2021 9,361 2 View
I think that most of us have met the phenomenon that even we use the totally same treatment to handle the mice with same bacground in our model preparation. So I wondering that is it meaningful...
19 November 2021 4,093 1 View
Dear fellows, Like what I have mentioned above, I am wondering how to predict a small molecular inhibitor candidate to a protein with known structure in PDB. Is there anying method to achieve...
22 September 2021 2,243 0 View
Recently, I want to verify the certain treatment enhancing on antigen presenting ability of macropohages. I searched the literitures, using OT.II T lymphocytes and OVA antigen, OT.1 lymphocyte...
07 September 2021 3,756 3 View
For the most cases, higher PD-L1 expression or lower PD-L1 expression is helpful for tumor cell itself to defense host immune?
11 January 2019 4,882 2 View
Recently, I have injected the cultured tumor cells derived from mouse to C57 background mouse by subcutaneous injection method, I can see the tumor mass formation. Now I wonder whether it is...
13 December 2017 4,543 4 View
we want to test a drug (a kind of protein with small molecular weight) on mice, and do not know how to chose the method of administration.
12 June 2017 6,491 4 View
In my recent experiment, I found that, compared with the control group, there are many kinds of IGKV segment mRNA up-regualted. What is the phenomenon indicates? dose it mean the experiment...
01 January 1970 3,390 0 View
