How do you interpret and understand 11B 3Q MAS NMR graph?

I am a novice to NMR and need help in understanding what i am looking at in terms of 11B 3Q MAS NMR graphs. Any resources for beginners would be helpful in interpreting the data. Literature only...

20 January 2021 2,648 3 View

Is there a way to distinguish between overlapping energies in XAS graphs from the P L1-edge from the B K-edge?

Is there a post analytical methodology or experimental technique used to distinguish between overlapping energy edges in XAS?

09 September 2020 2,894 4 View

How do you apply chemometrics to Raman spectroscopy of glass samples with varying degrees of crystallinity?

What are the formula's and peak fitting types needed e.g. gaussian, lorenzian...

28 April 2020 2,301 3 View

Is there any other analysis methods one can do to analyse Raman spectroscopy data, other than stacking the plots and doing difference curves?

I am looking at glasses and the effect a certain component has on the peak positions and intensities. I do not have any complimentary techniques as of yet to help me analyse the data such as NMR.

27 April 2020 256 5 View

How to interpret and analyse data from accelerometer and gyroscope?

Hello, I am a research student looking into behaviour of horses. I am trying to use IMU devices to detect movement in horse movement (step frequency, lying down, etc). I understand how the sensor...

07 August 2018 5,646 3 View

What is the ideal procedure for testing anti-tumor with low cost price? What can you suggest procedure for extracting polysaccharides?

Hi! I am Katrina Co from the Philippines! I am just a High School student, I have only a limited samples for testing and a low budget (maybe around 6000 pesos or around $120). I only have limited...

29 May 2018 8,311 3 View

Any ideas for a protocol or extraction kit for extracting DNA from tomato fruit for the study of bacterial microbiota using NGS?

I'm trying to extract DNA from tomato fruit in order to study the bacterial microbiota found inside the fruit using NGS. I have tried using a CTAB protocol, but the precipitate is highly charged...

20 April 2018 423 3 View

I am looking for the protocol for DNA extraction from prostate biopsies?

I am planning to use COBAS 4800 to test for HPV in prostate cancer. What will be the best sample type for DNA extraction and the best protocol?

20 November 2017 2,387 2 View

Can anyone suggest a psychometrically valid, reliable, and quantitative measure of "Intentionality" attributions?

20 May 2015 5,982 2 View

How to check if the primer can attach to another organism from the same family?

I am working with two types of viruses from the same family. I was provided with specifically designed primers to test if either of the viruses are present in the material. However, I ran a PCR...

02 May 2014 4,866 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View