Can I compare two mean scores of the same instrument, in the same sample? (Paired t-test)?

Hello everyone, I have scores on different scales of a sample of children, and I want to compare the mean scores on the different scales of this sample. For instance, my hypothesis is that...

10 December 2020 2,278 5 View

Protocol for proliferation of CD4+ murine T-cells?

23 July 2019 3,586 7 View

How to dissolve Dasatinib in order to go in vivo?

Dasatinib is an inhibitor of tyrosine kinases. I would like to test the effect of Dasatinib in vivo on my T cells. However, Dasatinib has a very low solubility (0.5mg/ml) with PBS, which is...

21 July 2019 3,056 3 View

Lysing of cell to detect antibody internalization?

07 April 2019 3,293 10 View

Fix 7-AAD staining?

Hi, I would like to make a viability staining using 7-AAD the day before the measurement; Is it possible to fix the cells? If yes, what do you suggest? Thank you!

01 April 2019 4,376 2 View

I need to measure the respiration rates in insects larvae. Do you have any suggestions about a feasible and not expensive equipment that can be used?

I know that there are avaialble some respiration chambers for insects but I was wondering if chambers used for small fruites can be adequate for the scope.

15 October 2017 3,205 2 View

What statistical analysis should I use for hierarchical questions data?

16 November 2015 9,532 4 View

Is it possible to change the orientation of hydrogen atoms of the ligand and protein residues? If yes, how is it possible by using Gromacs 5.0.4?

Dear users, I would like to change the orientation of an hydrogen of a D-serine (my ligand) and of a serine (as protein residue). How is it possible by using Gromacs 5.0.4? Thank you

12 September 2015 6,906 5 View

Could you please recommend me a manufacturer/ vendor of the GLAD(Glancing Angle Deposition) system?

Ideally we would like to buy a PVD equipment that can be used for the layer by layer and columnar growth.

09 June 2015 9,952 4 View

Can anyone suggest a free qPCR Data Analysis Software running on Mac?

Thanks a lot, in advance.

03 February 2015 4,087 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View