Can I compare two mean scores of the same instrument, in the same sample? (Paired t-test)?

Hello everyone, I have scores on different scales of a sample of children, and I want to compare the mean scores on the different scales of this sample. For instance, my hypothesis is that...

10 December 2020 2,278 5 View

Protocol for proliferation of CD4+ murine T-cells?

23 July 2019 3,586 7 View

How to dissolve Dasatinib in order to go in vivo?

Dasatinib is an inhibitor of tyrosine kinases. I would like to test the effect of Dasatinib in vivo on my T cells. However, Dasatinib has a very low solubility (0.5mg/ml) with PBS, which is...

21 July 2019 3,056 3 View

Lysing of cell to detect antibody internalization?

07 April 2019 3,293 10 View

OT-I Rag1-/- genotyping protocol?

Dear all, Does anyone have a protocol to provide me to genotype OT-I Rag1-/- mouse? I have taken a look to OT-I and Rag1-/- genotyping protocols and I would need 4 primers for Tcra, 4 for Tcrb and...

10 December 2018 2,449 2 View

I need to measure the respiration rates in insects larvae. Do you have any suggestions about a feasible and not expensive equipment that can be used?

I know that there are avaialble some respiration chambers for insects but I was wondering if chambers used for small fruites can be adequate for the scope.

15 October 2017 3,205 2 View

What statistical analysis should I use for hierarchical questions data?

16 November 2015 9,532 4 View

Is it possible to change the orientation of hydrogen atoms of the ligand and protein residues? If yes, how is it possible by using Gromacs 5.0.4?

Dear users, I would like to change the orientation of an hydrogen of a D-serine (my ligand) and of a serine (as protein residue). How is it possible by using Gromacs 5.0.4? Thank you

12 September 2015 6,906 5 View

Could you please recommend me a manufacturer/ vendor of the GLAD(Glancing Angle Deposition) system?

Ideally we would like to buy a PVD equipment that can be used for the layer by layer and columnar growth.

09 June 2015 9,952 4 View

Can anyone suggest a free qPCR Data Analysis Software running on Mac?

Thanks a lot, in advance.

03 February 2015 4,087 2 View

No title

02 March 2021 3,060 3 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View