07 December 2018 1 4K Report

I want to know what would be best to use for gel electrophoresis of a 3.3kb DNA fragment. I would like to perform a gel extraction and cloning with the fragment. What would be the best voltage using TE buffer?

More Melody Shumba's questions See All
How many seeds should be inoculated in 10 mL bacterial inoculum?

Can someone cite some published research regarding this matter? Well, I need to know the ideal ratio of number of seeds with 10mL bacterial inoculum.

01 February 2024 9,313 0 View

What media to use?

If I am testing the antimicrobial activity of a bacteria in a specific agar (like Nutrient Agar), shouldn't I use the same media for other tests such as biochemical analysis? I am a bit lost as...

03 January 2024 2,956 3 View

How does one install JupyterLab on a MacBook Air?

Greetings, For one of my cognitive sciences lab session, I have to install JupyterLab on my computer (a MacBook Air). However I really am lost with how to do it, especially since I'm not tech...

14 August 2023 4,135 2 View

Will you assist with suggesting peer reviewed articles for the initiation of telehealth ?

Will you assist with suggesting peer reviewed articles in support of the initiation of a telehealth platform in a managed care plan to improve the Transition of Care process when a member is...

27 May 2023 6,017 1 View

How can i fix this error in CST :unable to evaluate expression "r_in"?

Hi, I'm trying to create a cylinder by parameters like r_in as it's smaller radius...but it gives me the following error : unable to evaluate expression "r_in". (that's when I gave it...

05 April 2023 5,302 2 View

Are they research methodology using a continuous performance task coded using PsychoPy?

We are two third year bachelor of psychology students looking into using PsychoPy for a continuous performance task. (CPT) Our project is about the interactions between boredom proneness and...

13 December 2022 3,108 2 View

Does anyone know of any resting state EEG datasets for psychiatric disorders?

I an looking for an resting state (eyes closed) EEG datasets for any kind of psychiatric disorder. These can include, but not limited to Alcohol use disorder Acute stress disorder Addictive...

16 July 2022 7,432 2 View

How to download and display cloud data from CALIOP for the Arctic?

I need to download and display cloud data specially VFM from CALIOP . could you please help me with this issue? I need the amount of memory I need for that for about only 1-year of data. I already...

05 July 2022 2,016 0 View

Isolating keratinocytes from embryos with defective skin barrier?

Does anyone have a protocol for isolating keratinocytes from late stage embryos with defective skin barrier? The embryos we have have very thin, fragile skin making skin removal following dispase...

14 June 2019 4,905 0 View

ITO glass vs Soda-lime glass, which one is more hydrophilic?

ITO glass without any hydrophilicity treatment or cleaning, is it more hydrophilic than soda-lime glass? Thank you in advance!

23 October 2017 2,315 3 View

Similar questions and discussions
Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close