Optimum conditions for gel electrophoresis of DNA fragment that 3.3kb long?

07 December 2018 1 4K Report

I want to know what would be best to use for gel electrophoresis of a 3.3kb DNA fragment. I would like to perform a gel extraction and cloning with the fragment. What would be the best voltage using TE buffer?

Karen A. Darbinyan

Hello, use 1.6 % Agarose gel for 3.3kbp DNA and voltage 80-120V if your equipment current will allow.

Regards.

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Science method

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