Robert Flynn

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Questions related from Robert Flynn

Optimal method for RNA/mRNA reprogramming of primary human fibroblasts in order to obtain iPSCs?

I am working with primary human fibroblasts from patients with severe metabolic disease. I am interested in using RNA/mRNA re-programming of these fibroblasts in order to obtain iPSCs for...

17 January 2022 3,730 3 View

RNA-Sequencing in human primary fibroblasts: How do I control for biopsy site?

I am currently analysing some RNA-Seq data from human primary fibroblasts. I noticed in the following paper (https://www.nature.com/articles/ncomms15824) that the expression of hox genes was used...

16 December 2021 6,255 1 View

Any suggested alterations to the Zymo RNA Microprep kit to increase RNA purity?

I am currently performing RNA extractions from FACS sorted nuclei. The yields have been good. However, purity measures (i.e. 260/280, 260/230 ratios) have been poor. I suspect phenol contamination...

26 August 2020 2,916 2 View

Can anyone point me to a paper which could explain the increased prevalence of "epithelial like" SHSY5Y cells after differentiation?

Hi all, I have conducted a CRISPR knockout in SHSY5Y cells and have carried out a few differentiations on them. We do an 8 day protocol using 10uM retinoic acid and 1% FBS which is fairly...

24 July 2019 6,123 0 View

Is my technique at fault while loading gels for electrophoresis?

I recently completed a gradient PCR but my bands were much less than satisfactory. I have a nagging feeling that my pipetting technique is at fault when loading. I use standard forward pipetting...

18 December 2018 1,145 4 View