Is there any online tool available for predicting promoter and terminator sequences within the mRNA sequence?
Please have a look at this site:
http://molbiol-tools.ca/Promoters.htm
Regards, Christian
thank you for your reply, I tried a few tools on that website but it doesn't work..
When working with the prediction tools you need to enter the genomic DNA sequence. mRNA does not contain promoter or terminator sequences. These regions are needed for the correct transcription of DNA-encoded genes.
Cheers, Christian
You can check for promoter :http://www.fruitfly.org/seq_tools/promoter.html
and for terminator:http://rna.igmors.u-psud.fr/toolbox/arnold/index.php#Results
thanks
Is anyone familiar with conducting iron uptake assay for filamentous fungi? I am trying to measure the concentration of iron in growth media in order to know how much iron (Fe3+ or Fe2+) is...
07 August 2016 8,096 3 View
Anybody knows from where I can download the sequence of this gateway destination plasmid pK7GW1WG2(1).O? Thanks in advance
06 July 2016 1,937 2 View
TB3 is a common media used for the regeneration of protoplast.. Anybody knows what is it abbreviated for? If you have a reference for it, kindly share with me... Thank you
04 May 2016 1,389 1 View
I want to express a fungal gene under the control of it's own promoter but there is no reliable online tools to find out where exactly the promoter of that gene is, how many bases upstream or...
04 May 2016 784 7 View
After double digestion of plasmid with Xba1 and HindIII for 4 hours, I run it on gel but unexpectedly each lane was full of smears and the bands were not clearly visible. What can be the possible...
02 March 2016 8,598 9 View
Actually I'm transforming the AGL1 strain with pGKO2 containing our gene construct, but i don't know the reason why the Agrobacterium can't grown after electroporation with the plasmid? what can...
08 September 2015 7,274 5 View
We are trying to get a pure culture of Verticillium dahliae on PDA, but after sometime green color colonies of some other spores appear on the plate, when seen under microscope, the size is quite...
02 March 2015 3,655 17 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,405 2 View