05 May 2016 1 1K Report

TB3 is a common media used for the regeneration of protoplast.. Anybody knows what is it abbreviated for? If you have a reference for it, kindly share with me...

Thank you

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Iron Uptake Assay for Filamentous Fungi?

Is anyone familiar with conducting iron uptake assay for filamentous fungi? I am trying to measure the concentration of iron in growth media in order to know how much iron (Fe3+ or Fe2+) is...

07 August 2016 8,096 3 View

Where can i get the pK7GW1WG2(1).O plasmid sequence ?

Anybody knows from where I can download the sequence of this gateway destination plasmid pK7GW1WG2(1).O? Thanks in advance

06 July 2016 1,937 2 View

Expression of a fungal gene under the control of it's own promoter?

I want to express a fungal gene under the control of it's own promoter but there is no reliable online tools to find out where exactly the promoter of that gene is, how many bases upstream or...

04 May 2016 784 7 View

Online promoter and terminator prediction tool?

Is there any online tool available for predicting promoter and terminator sequences within the mRNA sequence?

02 March 2016 5,138 5 View

Why do i have smears on gel after restriction digestion with 2 enzymes?

After double digestion of plasmid with Xba1 and HindIII for 4 hours, I run it on gel but unexpectedly each lane was full of smears and the bands were not clearly visible. What can be the possible...

02 March 2016 8,598 9 View

If AGL1 Agrobacterium is transformed with pGKO2 vector without gene insert, can it grow on LB medium with kan antibiotics?

Actually I'm transforming the AGL1 strain with pGKO2 containing our gene construct, but i don't know the reason why the Agrobacterium can't grown after electroporation with the plasmid? what can...

08 September 2015 7,274 5 View

How can I control the growth of unwanted spores in the Verticillium dahliae culture?

We are trying to get a pure culture of Verticillium dahliae on PDA, but after sometime green color colonies of some other spores appear on the plate, when seen under microscope, the size is quite...

02 March 2015 3,655 17 View

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