8 Questions 31 Answers 0 Followers
Questions related from Latif ur Rehman
Is anyone familiar with conducting iron uptake assay for filamentous fungi? I am trying to measure the concentration of iron in growth media in order to know how much iron (Fe3+ or Fe2+) is...
08 August 2016 8,041 3 View
Anybody knows from where I can download the sequence of this gateway destination plasmid pK7GW1WG2(1).O? Thanks in advance
07 July 2016 1,886 2 View
TB3 is a common media used for the regeneration of protoplast.. Anybody knows what is it abbreviated for? If you have a reference for it, kindly share with me... Thank you
05 May 2016 1,346 1 View
I want to express a fungal gene under the control of it's own promoter but there is no reliable online tools to find out where exactly the promoter of that gene is, how many bases upstream or...
05 May 2016 750 7 View
After double digestion of plasmid with Xba1 and HindIII for 4 hours, I run it on gel but unexpectedly each lane was full of smears and the bands were not clearly visible. What can be the possible...
03 March 2016 8,556 9 View
Is there any online tool available for predicting promoter and terminator sequences within the mRNA sequence?
03 March 2016 5,078 5 View
Actually I'm transforming the AGL1 strain with pGKO2 containing our gene construct, but i don't know the reason why the Agrobacterium can't grown after electroporation with the plasmid? what can...
09 September 2015 7,233 5 View
We are trying to get a pure culture of Verticillium dahliae on PDA, but after sometime green color colonies of some other spores appear on the plate, when seen under microscope, the size is quite...
03 March 2015 3,621 17 View
