For an HPLC System, spectrum bandwidth mentioned as 8 nm. How to calculate this spectrum bandwidth?

Most of the HPLC Brochures/Specifications sheet mentions 'spectrum bandwidth' as 8 nm. But, how to calculate this one. Please suggest.

12 December 2019 4,770 3 View

Is there any Alternative for Sodium Naphthalene Sulfonate?

I am looking for alternate to SNF's (Dispersing Agent). The present one is in yellow colour and I am looking for the similar type of Dispersing agent in white colour.

29 August 2019 7,921 1 View

How to keep reference in predictor variable and outcome variable in multiple logistic regression analysis by using EPI INFO 7 Software?

HI, I am analyzing data related to a project entitled "Assessment of stigma, fear and discrimination among healthcare workers towards PLWHIV". We are planning to associate socio-demographic...

21 April 2019 1,615 2 View

Can we leverage Artificial Intelligence & Image Processing for Online Inspection of Packed Cases..?

TITLE: Leveraging Artificial Intelligence & Image Processing for Online Inspection of Packed Cases 1.BACKGROUND: Leaves of a crop are threshed and are packed into 200 Kg cases. 10% of these...

06 February 2019 8,322 2 View

Anyone using Flowing software for Flow cytometry data knows how to collect all Overlay histograms together in the Image collector?

I want to compare how the cell population is changing from time zero through the process, for which I want to see the chnage in the FSC histogram for each sample with respect to the time zero. I...

26 December 2018 2,566 3 View

How can i create a set of nodes during the analysis of model in abaqus?

22 January 2017 4,114 6 View

16S rRNA gene of bacteria is amplified but not able to sequence?

I was trying to sequence 16S rRNA gene of a bacteria. The gene is being amplified but unable to sequence it. Sequencing was attempted for 3 times but failed. What might be the reason?

27 May 2016 1,341 8 View

Does bacteria alter /adjust the pH of the liquid media itself while growing?

08 May 2016 887 15 View

Is there any Procedure to Prepare in situ preparation of CuO Nanoparticles inside PVA network.

I want to prepare CuO nanoparticles in side poly vinyl alcohol network. When ever i am trying to react copper ions in PVA solution with naOH, I am getting Green precipitate.This is copper...

07 March 2016 9,217 4 View

Fresh, decontaminated, hypo and UV treated wares and area being used as suggested. Still false positive appears in no template control, why?

I am using all the fresh working stocks, water, aerosol tips, changed the working room areas and further more decontaminated the working area with bleach and UV. Still than consistently false...

27 September 2015 2,698 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

No title

28 February 2021 9,936 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View