For an HPLC System, spectrum bandwidth mentioned as 8 nm. How to calculate this spectrum bandwidth?

Most of the HPLC Brochures/Specifications sheet mentions 'spectrum bandwidth' as 8 nm. But, how to calculate this one. Please suggest.

12 December 2019 4,770 3 View

Is there any Alternative for Sodium Naphthalene Sulfonate?

I am looking for alternate to SNF's (Dispersing Agent). The present one is in yellow colour and I am looking for the similar type of Dispersing agent in white colour.

29 August 2019 7,921 1 View

How to keep reference in predictor variable and outcome variable in multiple logistic regression analysis by using EPI INFO 7 Software?

HI, I am analyzing data related to a project entitled "Assessment of stigma, fear and discrimination among healthcare workers towards PLWHIV". We are planning to associate socio-demographic...

21 April 2019 1,615 2 View

Can we leverage Artificial Intelligence & Image Processing for Online Inspection of Packed Cases..?

TITLE: Leveraging Artificial Intelligence & Image Processing for Online Inspection of Packed Cases 1.BACKGROUND: Leaves of a crop are threshed and are packed into 200 Kg cases. 10% of these...

06 February 2019 8,322 2 View

Anyone using Flowing software for Flow cytometry data knows how to collect all Overlay histograms together in the Image collector?

I want to compare how the cell population is changing from time zero through the process, for which I want to see the chnage in the FSC histogram for each sample with respect to the time zero. I...

26 December 2018 2,566 3 View

How can i create a set of nodes during the analysis of model in abaqus?

22 January 2017 4,114 6 View

16S rRNA gene of bacteria is amplified but not able to sequence?

I was trying to sequence 16S rRNA gene of a bacteria. The gene is being amplified but unable to sequence it. Sequencing was attempted for 3 times but failed. What might be the reason?

27 May 2016 1,341 8 View

Does bacteria alter /adjust the pH of the liquid media itself while growing?

08 May 2016 887 15 View

Is there any Procedure to Prepare in situ preparation of CuO Nanoparticles inside PVA network.

I want to prepare CuO nanoparticles in side poly vinyl alcohol network. When ever i am trying to react copper ions in PVA solution with naOH, I am getting Green precipitate.This is copper...

07 March 2016 9,217 4 View

Fresh, decontaminated, hypo and UV treated wares and area being used as suggested. Still false positive appears in no template control, why?

I am using all the fresh working stocks, water, aerosol tips, changed the working room areas and further more decontaminated the working area with bleach and UV. Still than consistently false...

27 September 2015 2,698 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View