Narayana Ruthwek Mamillapalli

8 Questions 4 Answers 0 Followers

Questions related from Narayana Ruthwek Mamillapalli

How to calculate PIC value for SSR?

11 July 2022 4,140 2 View

What is the best and accurate method to calculate annealing temperature of primers?

Can someone give me formulas to calculate annealing temperature of primers accurately?

20 June 2022 8,609 3 View

What is the reason for these kind of bands of my PCR product?

I have done gel electrophoresis of my PCR product the results were shown like this there were no proper bands observed in the gel and some of the amplified DNA bands were stuck at the well region...

14 June 2022 2,512 3 View

What is the need for morpho physio biochemical analysis?

What is the need and importance of doing morphological physiological and biochemical analysis?

10 June 2022 6,771 1 View

How to analyse gel documentation photos using software programs?

Can someone explain me

10 June 2022 873 3 View

Can we dilute 2x PCR master mix or use it directly without any dilution ?

I have GeNei PCR Master Mix of 2x concentration shall I dilute it to 1x before using for PCR or use it directly without any dilution Can someone tell me what is the best way to use Master Mix

05 June 2022 1,744 5 View

What could be the reason for this type of bands DNA ?

I have done DNA isolation of sugarcane but it seems there is a problem in the bands(upper row) what could be the reason for this

30 April 2022 7,973 6 View

Why there are no DNA bands ?

I have done DNA isolation of sugarcane every time I don't get any bands of DNA can anyone tell me what could be the reason for this and why the bands are appearing like this?

27 April 2022 5,326 4 View