Nuclear E-cadherin

Diego Benitez Riquelme @Diego-Benitez-Riquelme

01 January 1970 1 6K Report

Hi everyone!

I was performing some immunofluorescence using SW480 colorectal cancer cell line and after KO of a specific gene (that we hypothesize it is a tumor suppressor), we observed nuclear E-cadherin like in the image.

There are some roles of nuclear E-cadherin already described but, is it possible to observe such pattern? have anyone some comments regarding nuclear E-cadherin or have you ever got similar results?

Thank you in advance!

Diego

Blue: Hoechst (nuclei)

Green: Phalloidin (F-actin)

Red: E-cadherin

Ellen A G Chernoff

I think your nuclear expression pattern has similarities with the one shown in this paper in which there is interaction of E-cadherin with CTNND1/β-catenin:

https://aacrjournals.org/mcr/article/17/2/655/90010/Nuclear-E-Cadherin-Acetylation-Promotes-Colorectal

Badges
Science topic

More Diego Benitez Riquelme's questions See All

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View

Which commercial technology is available ? 4 brain ?

Following https://catniplab.github.io/Tafazoli-LearningControlBrain/ and https://insights.princeton.edu/2020/05/learning-to-control-the-brain/ which commercial technology is available ? Probably...

15 July 2024 4,339 0 View

What could be causing these smears in my PCR electrophoresis gel?

I am new to running PCR gels. I loaded this gel and I thought it was fine, meaning I saw/felt no apparent punctures or spillovers to neighboring wells (see picture 1). When the gel started to run,...

30 June 2024 4,107 4 View

What are those white spots at the end?

I'been seeing those white spots at the end of my wet-transfer lately. Since the Ponceu Red doesn't stain I guess there isn't any protein. Since it's under the 42kDa mark It isn't a problem since I...

18 June 2024 1,734 0 View

Condensation of a Q8 serendipity FE to a Q4 FE with drilling DOFs?

Hello, Can somebody please give me some reference to a paper or book where it is explained how to condensate: * from a Q8 serendipity FE to a Q4 FE with drilling DOFs (16 DOFs to 12 DOFs). *...

05 June 2024 936 1 View

Laser for perovskite module?

Hi, I'd like to know if someone knows about a company where I can buy an affordable laser-scriber for perovskite films and another thin film for the fabrication of modules. Many thanks,

31 May 2024 2,841 0 View

Organoid with Claustro can be linked to LLM like chatGPT ?

Organoid with Claustro can be linked to LLM Neuroplatform - FinalSpark , finalspark.com/neuroplatform/ can be linked to chatGPT ? What to do with this ? Avatar Pilots ? Studying the case of...

28 May 2024 3,302 0 View

Tumor Organoid Growth ?

Hi everyone, I am culturing colorectal cancer organoids (Control and Experimental) and I observe a faster growing (number and size of organoids) in experimental condition. Now the question is,...

27 May 2024 9,258 0 View

Troubleshooting western blot for 200 kD Protein. Can anyone help?

The truth is that I have had many, many problems to be able to see a specific band of this protein. My problems have ranged from bad transfers, to unknowns that I have not been able to answer. The...

26 April 2024 8,595 4 View

Using a preform analyzer to measure the refractive index profile of other samples than silica preforms?

Hello I have to work with a preform analyzer model 2650 - Photon Kinetics, but I have no preforms yet to start the calibration and the tests. Is it possible use other kind of sample with a well...

25 April 2024 564 1 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

How Can I Resolve a Persistent Unwanted NMR Peak at 1.25 ppm?

Hello everyone, I am facing a consistent issue in my NMR spectra with an unwanted peak appearing at 1.25 ppm. This peak seems to vary with the amount of sample: it becomes more pronounced with...

15 July 2024 9,065 4 View

Is perfusion required to stain brain tissue with every method?

I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...

14 July 2024 177 4 View

Does anybody have Anti-Claudin8 and Anti-Claudin2 Primary conjugated Ab (Target Species: Human)?

I need Anti-Claudin8 Primary and Anti-Claudin2 conjugated Ab for my immunofluorescence experiment. If anybody has worked on it. Please share the accession number/details.

14 July 2024 485 0 View

What are the criteria that must be retained for the development and validation of a qualitative NMR method ?

Dear Community, I would like to develop and validate a qualitative NMR method for the analysis of a specific category of chemicals, and my question is the following: What are the criteria that I...

08 July 2024 1,402 4 View

Can I use BSA for a Histological Blocking Solution?

Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...

07 July 2024 7,967 3 View

?como elimino articulos que no son propios?

eliminoar articulos que son de tu autoria

05 July 2024 2,107 1 View