Diego Benitez Riquelme

7 Questions 9 Answers 0 Followers

Questions related from Diego Benitez Riquelme

Tumor Organoid Growth ?

Hi everyone, I am culturing colorectal cancer organoids (Control and Experimental) and I observe a faster growing (number and size of organoids) in experimental condition. Now the question is,...

28 May 2024 9,127 0 View

Why number of organoids can be different?

Hello everyone, I have the following question regarding the number of organoids in culture. I performed a seeding assay with mouse tumor colorectal organoids. I seeded 2000 cells (dissociated...

22 September 2023 7,892 1 View

How can I precipitate the proteins from cell culture media?

Hello everyone, I am trying to evaluate by western blotting proteins secreted by cells to the culture medium (in cell cultures). I found a precipitation protocol with TCA/Acetone...

21 February 2022 9,041 9 View

Using expired transwell inserts?

Dear Researchers, In the Lab I have transwell inserts to do migration and invasion assays but the expiration date was october 2020. Do you think it is possible to use them and do not get altered...

08 July 2021 9,541 7 View

Lentiviral Infection with pX459 vector?

Hi everyone! Does anyone know if it is possible doing lentiviral infection with the vector pX459 ( pSpCas9(BB)-2A-Puro (PX459) V2.0)? I am asking because I infected iMEF cells with lentiviral...

29 January 2020 3,981 0 View

How can I confirm the correct insertion of sgRNA into pX459 vector?

Hi, I have already cloned a sgRNA into pX459 vector (pSpCas9(BB)-2A-Puro (PX459) V2.0) and now I have to sequence to confirm the correct insertion, but I do not know if there are specific...

19 January 2020 2,572 2 View

Cloning sgRNA into pX459 vector ?

Hi everyone! I am trying to clone a sgRNA into a pX459 but at the end I do not have colonies. To anneal the oligos I use sgRNA fwd (100uM) 1uL sgRNA Rev (100uM) 1uL 10x T4 Ligation Buff...

13 December 2019 7,910 12 View