02 September 2020 0 3K Report

Hi guys!

I'm trying to perform NP-KLH injection for the first time. It seems many protocols recommend using the mixture of NP-KLH with alum or Freud's adjuvant. Due to my inexperience in this area, I'm bit concerned for making this mixture.

- Do I just add alum in NP-KLH solution? (1:1 ~ 1:3 ratio)

- How long should I incubate & mix the alum? Should I use laboratory shaker ?

any help would greatly appreciated.

Thank you!!

More Joo Sung Gabriel Shim's questions See All
How many journal articles should come out of a doctoral thesis?

Is it normal if I publish my doctoral thesis into only one journal article (since my doctoral thesis will be data-heavy and theory-light)? Or are doctoral theses expected to be equivalent to 3-4...

01 December 2020 3,803 14 View

Why is my Simscape model of a Furuta pendulum not fully controllable?

Dear community, I am trying to build a model of a Furuta pendulum in Simulink/Simscape. Unfortunately, when I try to linearize my model with the integrated Model Linearizer, I get an unexpected...

20 August 2020 4,853 6 View

How do I perform image Anomaly detection?

I have 99 images like scratch and I have a part - blurred scratch image How do I detect a part - blurred image? I tried one-class SVM and Isolation Forest but they couldn't detect

19 May 2020 8,359 1 View

How can I develop linker on working electrode of screen printed electrode?

Hello all, We are using screen printed gold electrode(220BT) from Dropsens to build an immunosensor. To attach the antibody onto the working electrode, we use EDC/NHS with MPA. From what I...

10 February 2020 2,570 3 View

Non-specific optical stimulation of parvalbumin (PV) neurons - Any possible causes?

Hi all. Recently, I encountered a problem regarding the optogenetic stimulation experiments. In our study, we are using the blue laser (473 nm) to optogenetically stimulate parvalbumin (PV)...

05 February 2020 8,253 11 View

How can I calculate band gap of nano metal particle?

I'm studying for nano metal particles. The metal particles diameter is about 5nm. And the particles are stuck on nano wires through UV irradiation. I mean the nano wires are soaked in metal...

08 January 2020 8,807 6 View

Bicrystal fabrication company?

Hello. I am looking for a bicrystal fabrication company. My major is focusing on the exploration of grain boundary structure. And thus for a model experiment I need metal*or ceramic**...

08 January 2020 4,038 3 View

Does anyone have experience in using Doric Neuroscience Studio for fiber photometry experiments?

Hi. I am a graduate student using Doric Neuroscience Studio. Since there seems to be not much references about using Doric's fiber photometry console(FPC) (v. 4.x or 5.0.0) and our lab doesn't...

27 June 2019 9,512 2 View

Can fish succesfully survive in algal culture medium (e.g. CB)?

Yes, I'd like to conduct a experiment for co-cultivate both Microcystis and Zebra fish. I think fish will not be affected by algal culture media, but it's not clear. I'll be very appreciated, if...

30 October 2018 6,757 2 View

Is codon optimization necessary for human TDP 43 expression in Drosophila melanogaster?

Dear specialists in TDP 43 researches in Drosophila Melanogaster I actually constructed human TDP 43 in pacu2 vector(uas promoter included) and injected it into Drosophila. and checked how it...

13 October 2018 2,149 1 View

Similar questions and discussions
Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Translating two biomarkers to the same scale??

Hello researchers, I was wondering if anyone had experience in the following: I would like to translate two separate continuous biomarkers (A and B), that are mechanistically linked to each...

25 January 2021 2,107 3 View

What time and speed should the centrifuge be set at in order for cell debris and clumped proteins to form a pellet?

- The Eppendorf tube was then placed in a microcentrifuge. Notice how it is balanced with another tube placed opposite it in the centrifuge drum to ensure smooth rotation. Other information...

24 January 2021 2,547 3 View

Is Handheld XRF analyzer useable? and accurate in geochemical test?

What is the different between Lab XRF and Hanheld XRF, is it accurate and useable to analyze sedimentary rocks?

23 January 2021 9,941 6 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close