How many journal articles should come out of a doctoral thesis?

Is it normal if I publish my doctoral thesis into only one journal article (since my doctoral thesis will be data-heavy and theory-light)? Or are doctoral theses expected to be equivalent to 3-4...

01 December 2020 3,803 14 View

Why is my Simscape model of a Furuta pendulum not fully controllable?

Dear community, I am trying to build a model of a Furuta pendulum in Simulink/Simscape. Unfortunately, when I try to linearize my model with the integrated Model Linearizer, I get an unexpected...

20 August 2020 4,853 6 View

How do I perform image Anomaly detection?

I have 99 images like scratch and I have a part - blurred scratch image How do I detect a part - blurred image? I tried one-class SVM and Isolation Forest but they couldn't detect

19 May 2020 8,359 1 View

How can I develop linker on working electrode of screen printed electrode?

Hello all, We are using screen printed gold electrode(220BT) from Dropsens to build an immunosensor. To attach the antibody onto the working electrode, we use EDC/NHS with MPA. From what I...

10 February 2020 2,570 3 View

Non-specific optical stimulation of parvalbumin (PV) neurons - Any possible causes?

Hi all. Recently, I encountered a problem regarding the optogenetic stimulation experiments. In our study, we are using the blue laser (473 nm) to optogenetically stimulate parvalbumin (PV)...

05 February 2020 8,253 11 View

How can I calculate band gap of nano metal particle?

I'm studying for nano metal particles. The metal particles diameter is about 5nm. And the particles are stuck on nano wires through UV irradiation. I mean the nano wires are soaked in metal...

08 January 2020 8,807 6 View

Bicrystal fabrication company?

Hello. I am looking for a bicrystal fabrication company. My major is focusing on the exploration of grain boundary structure. And thus for a model experiment I need metal*or ceramic**...

08 January 2020 4,038 3 View

Does anyone have experience in using Doric Neuroscience Studio for fiber photometry experiments?

Hi. I am a graduate student using Doric Neuroscience Studio. Since there seems to be not much references about using Doric's fiber photometry console(FPC) (v. 4.x or 5.0.0) and our lab doesn't...

27 June 2019 9,512 2 View

Can fish succesfully survive in algal culture medium (e.g. CB)?

Yes, I'd like to conduct a experiment for co-cultivate both Microcystis and Zebra fish. I think fish will not be affected by algal culture media, but it's not clear. I'll be very appreciated, if...

30 October 2018 6,757 2 View

Is codon optimization necessary for human TDP 43 expression in Drosophila melanogaster?

Dear specialists in TDP 43 researches in Drosophila Melanogaster I actually constructed human TDP 43 in pacu2 vector(uas promoter included) and injected it into Drosophila. and checked how it...

13 October 2018 2,149 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View