Longest culture time for intracellular cytokine staining?

I want to incubate human CD4 T memory cells for 48 to dterminate cycle entry and proliferation. I plan to stimulate them for 48 hours. Can I add brefeldin for the last hors of this incubation in...

05 June 2019 612 4 View

Is making a start-up the only way to transfer technology?

My Institution just applied for a patent based on a finding of my group (we are based in Mexico). I want to promote its licensing to some company that might be interested. Has someone done this?...

10 November 2018 7,627 7 View

What kit for intracellular NAD determination do you recommend?

I need to measure intracellular NAD (total and NAD+) in human T cells. I see there are several systems (colorimetric and ELISA), and I need a kit that is reliable. I don't want to use HPLC for...

07 August 2018 7,150 1 View

CD4+ T cell lines expressing and not expressing CD38?

08 September 2017 9,020 2 View

What is the difference between Myc and cMyc tag proteins?

We are going to clone a protein in a pCMV-Myc plasmid. Looking for fluorochrome-conjugated antibodies to track the efficiency of transfection, I found both cMyc and Myc antibodies. I don't know...

08 September 2016 2,345 4 View

What online resources do you recommend for teachin immunology?

I am teaching a general immunology undergraduate course to students of laboratory medicine and pharmacology. I am looking for images, diagrams, text, and things of the sort as a support to my classes.

07 August 2016 833 3 View

What is the best LPS to induce inflammation in mice by ip injection?

LPS is obtained from different bacteria, and their activities may differ. Additionally the degree of glycosilation also influences LPS activity. Which origin or brand or kind of LPS yields a clear...

04 May 2016 2,058 6 View

Are there accepted markers of cell-cycle arrest?

We are looking for molecules whose presence is unique to arrest in G2 or M phases of cells cycle, whether as a result or as a cause of this arrest. We need to detect a molecule evidencing arrest...

31 December 2014 319 7 View

Can the maximal expression in an experiment be used as reference delta cT to calculate deltaDelta cT (Instead of the basal expression)?

10 November 2014 3,139 5 View

What's best to determine cell division (proliferation) by FACS, CFSE or PKH26?

I want to decide which dye to use to detect actual mitosis (not just DNA syntesis or cycling) in a highly purified subset of T cells. Does anyone has experience using both CFSE and PKH26? What's...

08 September 2014 7,830 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

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03 March 2021 5,360 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View