Which imidazole concentration do you use for protein- NiNTA elution?

We use 300 mM, but i have seen protocols using 500 mM.

Wouldn´t it be better, in theory, to use higher (>1/2 M) concentrations of imidazole to improve competetive elution from the NiNTA resin?

More David Scherf's questions See All
Similar questions and discussions